Zoological Studies

Vol. 57, 2018

(update: 2018.09.28; 11.07) 

Comprehensive Analysis of the Jellyfish Chrysaora pacifica (Goette, 1886) (Semaeostomeae: Pelagiidae) with Description of the Complete rDNA Sequence

Jinho Chae1, Yoseph Seo2, Won Bae Yu2, Won Duk Yoon3, Hye Eun Lee4, Soo-Jung Chang5, and Jang-Seu Ki2,*

1Marine Environmental Research and Information Laboratory, Gunpo 15850, Korea. E-mail: jinhochae@gmail.com
2Department of Biotechnology, Sangmyung University, Seoul 03016, Korea. E-mail: akdldytpq12@gmail.com (Seo); wonbae511@nate.com (Yu)
3Human and Marine Ecosystem Research Laboratory, Gunpo 15850, Korea. E-mail: wondukyoon@humer.co.kr
4Ocean Climate and Ecology Research Division, National Institute of Fisheries Science, Busan 46083, Korea. E-mail: pandalus97@gmail.com
5Fisheries Resources and Environment Division, West Sea Fisheries Research Institute, National Institute of Fisheries Science, Incheon 22383, Korea. E-mail: sjchang7@korea.kr (Chang)

(Received 5 April 2018; Accepted 24 September 2018; Communicated by James D. Reimer)

Jinho Chae, Yoseph Seo, Won Bae Yu, Won Duk Yoon, Hye Eun Lee, Soo-Jung Chang, and Jang-Seu Ki (2018) The Scyphomedusae genus Chrysaora consists of highly diversified jellyfishes. Although morphological systematics of the genus has been documented over the past century, characterization of molecular taxonomy has been attempted only recently. In the present study, we sequenced an 8,167 bp region, encompassing a single ribosomal DNA (rDNA) repeat unit, from Chrysaora pacifica, and used it for phylogenetic analyses. The tandemly repeated rDNA units turned out to consist of both coding and noncoding regions, whose arrangement was found to be the same as that of a typical eukaryote. None of the 5S rRNA sequences were found among the repeat units. Comparative analyses of jellyfish rDNA sequences showed that the 28S locus is highly informative and divergent compared to the 18S locus. Phylogenetic analyses of the 18S and 28S loci revealed that the Semaeostomeae order of jellyfish is separated into taxonomic groups by families and genera, with a few exceptions. The family Pelagiidae was in a clade separate from other groups, thus forming a monophyletic lineage. All Chrysaora included here formed a strongly supported clade within the family Pelagiidae, and Pelagiidae manifested a sister relationship with Cyanea. Nonetheless, Chrysaora was found to be paraphyletic in both 18S and 28S phylogenies. Chrysaora pacifica was clearly distinct from close relatives C. melanaster and C. quinquecirrha. These results provide a special reference for the DNA taxonomy of Pelagiidae jellyfishes in terms of nuclear cistron rDNA sequences and improve our understanding of the molecular phylogenetic relationships among Semaeostomeae jellyfishes.

Key words: Jellyfish, Pelagiidae, Chrysaora pacifica, Ribosomal DNA, Transcription repeat unit.

*Correspondence: Tel: +82-2-2287-5449. Fax: +82-2-2287-0070. E-mail: kijs@smu.ac.kr

Supplementary Materials: Table S1