Vol. 59, 2020
Molecular Approach to the Identification of Three Closely Related Slugs Deroceras Species (Gastropoda: Eupulmonata: Agriolimacidae)
Kamila S. Azjąc1,* and Daniel Stec2
of Environmental Sciences, Faculty of Biology, Jagiellonian University,
Gronostajowa 7, 30-387 Kraków, Poland. *Correspondence:
2Institute of Zoology and Biomedical Research, Faculty
of Biology, Jagiellonian University, Gronostajowa 9, 30-387 Kraków,
Poland. E-mail: firstname.lastname@example.org (Stec)
Received 27 July 2020 / Accepted 6 September 2020
Communicated by Benny K.K. Chan
Some species of slugs belonging to the genus Deroceras
are invasive and cause severe agricultural damage. Despite extensive
knowledge about their invasiveness, data on molecular differentiation
of these morphologically similar species are lacking. Here we present a
molecular approach to the identification of three closely related
species of the genus Deroceras - D. agreste (L., 1758), D. reticulatum (O. F. Müller, 1774) and D. turcicum (Simroth, 1894) (Gastropoda: Eupulmonata: Agriolimacidae) based on sequences of multiple molecular markers: cytochrome c oxidase subunit I (COI), cytochrome b (cyt-b),
internal transcribed spacer 2 (ITS-2) and 28S ribosomal RNA (28S rRNA).
We also provide detailed photomicrographs of the penis and penial gland
of the three species as it is the latter that holds the most important
phenotypic characters for distinguishing between these taxa. Since
identification of the studied species, based solely on morphology is
considered challenging, contributing a means of molecular
differentiation will aid further ecological and biodiversity surveys of
these important pests.
Key words: Taxonomy, Deroceras, Barcoding, Gastropods, Slugs.
Citation: Azjąc KS, Stec D. 2020. Molecular approach to the identification of three closely related slugs Deroceras species (Gastropoda: Eupulmonata: Agriolimacidae). Zool Stud 59:0oo. doi:-.
Materials: Table S1 | Table S2 | Table S3 | Table S4