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Vol. 36 No. 1, 1997
Short Note
Efficient Methods for Isolating Mitochondrial DNA from Fresh
or Fixed Molluscan Specimens
Ling Jiang1, Wen-Lung Wu2,* and Yao-Sung Lin1
1Dept. of Zoology, National Taiwan Univ., Taipei, Taiwan 106
2lnst. of Zoology, Academia Sinica, Taipei, Taiwan 115
Ling Jiang, Wen-Lung Wu and Yao-Sung Lin (1997)
Fast and efficient methods of isolating mtDNA from molluscan specimens
with different conditions have been developed. The standard method
involves isolation and purification of a large amount of mtDNA to a
moderate purity from regular samples, even these containing jelly,
mucus, or lipids; the 2nd method extracts mtDNA from a
small quantity of sample, such as from 1 trochophore; and the 3rd
method obtains mtDNA from formalin- or alcohol-fixed specimens.
Key words: Mollusks, mtDNA, Isolation.
*Correspondence: Tel: 886-2-7899553, Fax: 886-2-7899547, E-mail: zomalacolg@ccvax.sinica.edu.tw
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