Zoological Studies

Vol. 36 No. 1, 1997

Short Note

Efficient Methods for Isolating Mitochondrial DNA from Fresh or Fixed Molluscan Specimens

Ling Jiang1, Wen-Lung Wu2,* and Yao-Sung Lin1

1Dept. of Zoology, National Taiwan Univ., Taipei, Taiwan 106
2lnst. of Zoology, Academia Sinica, Taipei, Taiwan 115

Ling Jiang, Wen-Lung Wu and Yao-Sung Lin (1997) Fast and efficient methods of isolating mtDNA from molluscan specimens with different conditions have been developed. The standard method involves isolation and purification of a large amount of mtDNA to a moderate purity from regular samples, even these containing jelly, mucus, or lipids; the 2nd method extracts  mtDNA from  a small quantity of  sample, such as from 1 trochophore; and the 3rd method obtains mtDNA from formalin- or alcohol-fixed specimens.

Key words: Mollusks, mtDNA, Isolation.

*Correspondence: Tel: 886-2-7899553, Fax: 886-2-7899547, E-mail: zomalacolg@ccvax.sinica.edu.tw