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Vol. 36 No. 3, 1997
LH-lmmunoreactivity
and Ultrastructural Changes in the Pituitary Gland of Post-castrated
Rats: Quantitative and Morphologic Studies
TShang-Ming Yu1,2,*, Sung-Ling Yu1, Mei-Miao Chiu2 and Kwan-Hwa Lin3
1lnstitute
of Anatomy and 2Department of Anatomy, National Yang-Ming University,
Taipei, Taiwan 112, R.O.C. Tel: 886-2-8267071 or 8212301 ext 7071. Fax:
886-2-8263711 or 886-2-8212884. E-mail: smy@ym.edu.tw
3School of Physical Therapy, National Taiwan University, Taipei, Taiwan 100, R.O.C.
Shang-Ming Yu, Sung-Ling Yu, Mei-Miao Chiu and Kwan-Hwa Lin (1997)
LH-lmmunoreactivity and ul trastructural changes in the pituitary
gland of post-castrated rats: quantitative and morphologic studies. Zoological Studies 36(3):
230-239. At 1-d postcastration of 2-mo-old rats, LH-immunoreactive (IR)
cells were not hypertrophied in the female pituitary gland but a few
LH-IR cells were hypertrophied in that of males At 2-d
postcastration, a few LH-IR cells were hypertrophied in the female
pituitary gland and contained small cytoplasmic vacuoles in males. At
4-d postcastration, a few hypertrophied LH-IR cells contained a few
small cytoplasmic vacuoles in the female pituitary gland and contained
coalesced vacuoles in males. At 7-d post castration, numerous
hypertrophied LH-IR cells contained a few small cytoplasmic vacuoles in
the female pituitary gland and contained coalesced vacuoles in males.
At 14-d postcastration, a large number of hyper trophied LH-IR cells
contained numerous cytoplasmic vacuoles in the female pituitary gland
and had become arranged in irregular, interconnecting cords in males.
At the electron microscopic level, cisternae of the rough endoplasmic
reticulum (RER) were slightly dilated in non-hypertrophied gonadotrophs
of the female pituitary gland but moderately dilated in hypertrophied
gonadotrophs of males at 1-d postcastration. At 2-d postcastration,
cisternae of the RER were slightly dilated in hypertrophied
gonadotrophs of the female pituitary gland and moderately dilated in
those of males. At 4-d postcastration, cisternae of the RER were
moderately dilated in hypertrophied gonadotrophs of the female
pituitary gland and had coalesced in those of males At 7-d
postcastration, cisternae of the RER remained moderately dilated in
hypertrophied gonadotrophs of the female pituitary gland and had
coalesced and markedly dilated in those of males. At 14-d
postcastration, cisternae of the RER were similar to those in
hypertrophied gonadotrophs of female and male pituitary glands at 7-d
postcastration. Most of the secretory granules were small in size but a
few large ones were also present in the cytoplasm of female and male
hypertrophied gonadotrophs.
Key words: Castration, lmmunocytochemistry, LH (luteinizing hormone), Pituitary, Morphometry.
*Correspondence:
lnstitute of Anatomy and 2Department of Anatomy, National Yang-Ming
University, Taipei, Taiwan 112, R.O.C. Tel: 886-2-8267071 or 8212301
ext 7071. Fax: 886-2-8263711 or 886-2-8212884. E-mail: smy@ym.edu.tw
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