Zoological Studies

Vol. 38 No. 3, 1999

Expression of LDH-C Isozyme among Lizard Taxa: Evolutionary Implications for the Vertebrate Lactate Dehydrogenase Gene Family

Chien-Hsien Kuo1,2, San Kao2, Ching-Feng Weng1 and Sin-Che Lee1,*

1Institute of Zoology, Academic Sinica, Taipei, Taiwan 115
2Department of Biology, National Taiwan Normal University, Taipei, Taiwan 116

Chien-Hsien Kuo, San Kao, Ching-Feng Weng and Sin-Che Lee (1999) In order to more completely understand the complete basis for the multiple LDH isozymes in lizards, seven species of Taiwanese lizards belonging to 4 families and 7 genera were sampled. Starch gel electrophoretic patterns of lizard LDH isozymes from brain, eye, heart, liver, muscle, and testis were analyzed. Like all other vertebrates, lizards possess the 2 fundamental LDH loci—A and B. A 3rd locus, LDH-C, was detected only in testes of Hemidactylus frenatus, Eumeces elegans, and Mabuya longicaudata. This testisspecific product, designated according to tissue-specific expression exhibited, a faster anodal migration than did the other LDH isozymes. These findings suggest that the testis-specific LDH isozyme was derived from ancestor amniote LDH-A.

Key words: Lizard, Lactate dehydrogenase, Evolution, Amniote.

*Correspondence: -