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Vol. 39 No. 1, 2000
Universal Primers for Amplification of the Complete Mitochondrial 12S rRNA Gene in Vertebrates
Hurng-Yi Wang1,2, Mung-Pei Tsai2, Ming-Chung Tu1 and Sin-Che Lee2,*
1Department of Biology, National Taiwan Normal University, Taipei, Taiwan 106
2Institute of Zoology, Academia Sinica, Taipei, Taiwan 115
Hurng-Yi Wang, Mung-Pei Tsai, Ming-Chung Tu and Sin-Che Lee (2000) The conserved regions of tRNAPHE and 16S rRNA
in the vertebrate mitochondrial genome were compared in order to design
the primers, 12SR and 12SL. These universal primers can be broadly used
to amplify a 1.3-kb DNA fragment by polymerase chain reaction (PCR)
over a wide range of major vertebrate lineages represented by the
species listed in the text. There is little length variation of the PCR
product among different taxa. Further sequence analysis revealed that
the fragment contains complete lengths of 12S rRNA and tRNAVAL, and that the length of 16S rRNA
is 200 bp. In tests through all representative taxa investigated, the
above 2 primers could amplify the complete 12S rRNA gene from all
representative taxa investigated. As the 12S rRNA gene is widely used
for phylogenetic analyses among different hierarchies, the use of these
primer sets for study of higher-category phylogenies in vertebrates now
becomes possible.
Key words: 12SR and 12SL, Primers, PCR amplification, Vertebrates.
*Correspondence: Tel: 886-2-27899520. Fax: 886-2-27858059. E-mail: sclee@gate.sinica.edu.tw
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