Vol. 40 No. 2, 2001
Phylogeographic Structure of the Formosan Wood Mouse, Apodemus semotus Thomas
Fu-Hsiung Hsu1,3,*, Fei-Jann Lin2 and Yao-Sung Lin3
1Taiwan Endemic Species Research Institute, Council of Agriculture, Chichi, Nantou, Taiwan 552
2Institute of Zoology, Academia Sinica, Taipei, Taiwan 115
3Department of Zoology, National Taiwan University, Taipei, Taiwan 106
Fu-Hsiung Hsu, Fei-Jann Lin and Yao-Sung Lin (2001) Phylogeographic structure of the Formosan wood mouse, Apodemus semotus
Thomas (Muridae), was studied by the PCR-RFLP method. In total, 271
individuals collected from 23 locations throughout its distribution
range in Taiwan were examined. Eleven restriction enzymes were used to
assay restriction fragment length polymorphism in a 2800-bp PCR-
amplified fragment of mtDNA, representing part of the cytochrome b (CYTb) and the control region (DL).
In total, forty-four mtDNA haplotypes were detected. The sequence
divergence between all pairs of haplotypes ranged between 0.20% and
3.20%. Phylogenetic analysis revealed that the 4 primary mtDNA lineages
have been separated by sequence divergences of 0.86% to 1.78%. The
geographic structure of mtDNA diversity and the lineage distribution
are complex, but each lineage reveals a limited geographic distribution
from north to south in the Central Mountain Range of Taiwan. According
to the geographic distribution of 7 dominant haplotypes and the
locality-based UPGMA dendrogram, we resolved the northern and
south-central geographical assemblages. This phylogeographic pattern of
the species might have resulted from a plausible historical scenario
involving isolation of its populations in several intermontane refugia,
and their re-dispersion and introgression in postgla- cial periods
during the Pleistocene.
Key words: Phylogeographic structure, mtDNA, RFLP, Apodemus semotus.
*Correspondence: Tel: 886-4-92761331 ext. 141. Fax: 886-4-92761583.