Vol. 46 No. 6, 2007
The Influence of Volatiles from the Hindgut of the Pine
Sawyer, Monochamus alternatus (Coleoptera:
Cerambycidae), on Its Oviposition Behavior
Li1,2 and Zhong-Ning Zhang2,*
of Chemistry and Environmental Engineering, Yangtze University,
Jingzhou, Hubei Province 434023, China
2State Key Laboratory of Integrated Management of Pest
Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences,
Beijing 100080, China
Li and Zhong-Ning Zhang (2007) The oviposition behavior and
response of Monochamus alternatus females to bolts treated with hexane
extracts of the hindgut contents of M. alternatus females and males
were investigated in the laboratory. Females gnawed a significantly
smaller number of oviposition scars on bolts i.e., cross-sections of
trunks of Pinus massoniana treated with a hexane extract of the hindgut
contents of M. alternatus females than on control bolts. The number of
eggs deposited on bolts treated with the hexane extract of female
hindgut contents was also significantly fewer than on control bolts.
The number of scars made and eggs laid on bolts treated with the hexane
extract of male hindgut contents did not significantly differ from
those on control bolts. These results suggest the presence of a
putative oviposition deterrent in the hindgut contents of M. alternatus
females. Hexane extracts of the hindgut contents of both sexes were
analyzed by gas chromatography-mass spectrometry; α-pinene, β-pinene,
myrcene, 3-carene, limonene, terpinolene, and butylated hydroxytoluene
were identified in the contents of both sexes, while p-vinylguaiacol
was found only in females. Experiments with synthetic mixtures revealed
that a mixture of p-vinylguaiacol and butylated hydroxytoluene
exhibited oviposition-deterring activity.
Key words: Monochamus
alternatus, Oviposition deterrent, Hindgut, Pinus massoniana, Volatiles.
*Correspondence: Tel: 86-10-62612241. Fax: 86-10-62565689.