Zoological Studies

Vol. 47 No. 3, 2008

Construction of an Indian Muntjac BAC Library and Production of the Most Highly Dense FISH Map of the Species

Chyi-Chyang Lin1, Pei-Ching Hsu1,2, Tzai-Shiuan Li1, Shu-Ju Liao2, Ya-Ming Cheng1, Lie-Jiau Hsieh1, and Yueh-Chun Li 2,*

1Department of Medical Research, China Medical University Hospital, Taichung 402, Taiwan
2Department of Biomedical Sciences, Chung-Shan Medical University, 110 Sec. 1, Jianguo N. Rd., Taichung 402, Taiwan

Chyi-Chyang Lin, Pei-Ching Hsu, Tzai-Shiuan Li, Shu-Ju Liao, Ya-Ming Cheng, Lie-Jiau Hsieh, and YuehChun Li (2008) Following completion of the genome sequences of some mammalian species, comparative genomic studies in mammals have been actively conducted to assess gene changes or to identify syntenic conservation during evolution.  The Indian muntjac (Muntiacus muntjak vaginalis) (2n = 6 in the female and 7 in the male) may have evolved from an ancient deer species with a karyotype 2n = 70 through extensive chromosome rearrangements creating the lowest chromosome number of a mammalian species.  Therefore, the species has become a good resource for studying syntenic conservation among deer species.  An Indian muntjac bacterial artificial chromosome (BAC) library that contains 126,336 individual BAC clones with an average insert size of 80 kilobases was obtained in this study.  The frequency of clones with inserts was 88%, and thus this library corresponds to approximately 4x coverage of the Indian muntjac genome.  Individual chromosomal locations of 1619 BAC clones on the Indian muntjac metaphase chromosomes were identified by fluorescence in situ hybridization (FISH).  Among these clones, 1517 BAC clones were mapped onto specific loci, and 102 BAC clones were mapped onto the centromeric region.  This provides the most highly dense FISH BAC clone map for the species.  This densely ordered map can be used as a blueprint for comparative FISH mapping studies of other deer species in order to investigate the mechanism of genomic rearrangement and karyotypic evolution.  Moreover, centromeric BAC clones will provide an excellent resource for studying the structure and function of mammalian centromeres.

Key words: BAC library, FISH mapping.

*Correspondence: Tel: 886-4-24730022 ext 11814.  Fax: 886-4-23248187.  E-mail:ycl@csmu.edu.tw