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Vol. 47 No. 3, 2008
Construction of an Indian Muntjac BAC Library and Production of the Most Highly Dense FISH Map of the Species
Chyi-Chyang Lin1, Pei-Ching Hsu1,2, Tzai-Shiuan Li1, Shu-Ju Liao2, Ya-Ming Cheng1, Lie-Jiau Hsieh1, and Yueh-Chun Li 2,*
1Department of Medical Research, China Medical University Hospital, Taichung 402, Taiwan
2Department of Biomedical Sciences, Chung-Shan Medical University, 110 Sec. 1, Jianguo N. Rd., Taichung 402, Taiwan
Chyi-Chyang Lin, Pei-Ching Hsu, Tzai-Shiuan Li, Shu-Ju Liao, Ya-Ming Cheng, Lie-Jiau Hsieh, and YuehChun Li (2008) Following
completion of the genome sequences of some mammalian species,
comparative genomic studies in mammals have been actively conducted to
assess gene changes or to identify syntenic conservation during
evolution. The Indian muntjac (Muntiacus muntjak vaginalis) (2n = 6 in the female and 7 in the male) may have evolved from an ancient deer species with a karyotype 2n
= 70 through extensive chromosome rearrangements creating the lowest
chromosome number of a mammalian species. Therefore, the species
has become a good resource for studying syntenic conservation among
deer species. An Indian muntjac bacterial artificial chromosome
(BAC) library that contains 126,336 individual BAC clones with an
average insert size of 80 kilobases was obtained in this study.
The frequency of clones with inserts was 88%, and thus this library
corresponds to approximately 4x coverage of the Indian muntjac
genome. Individual chromosomal locations of 1619 BAC clones on
the Indian muntjac metaphase chromosomes were identified by
fluorescence in situ
hybridization (FISH). Among these clones, 1517 BAC clones were
mapped onto specific loci, and 102 BAC clones were mapped onto the
centromeric region. This provides the most highly dense FISH BAC
clone map for the species. This densely ordered map can be used
as a blueprint for comparative FISH mapping studies of other deer
species in order to investigate the mechanism of genomic rearrangement
and karyotypic evolution. Moreover, centromeric BAC clones will
provide an excellent resource for studying the structure and function
of mammalian centromeres.
Key words: BAC library, FISH mapping.
*Correspondence: Tel: 886-4-24730022 ext 11814. Fax: 886-4-23248187. E-mail:ycl@csmu.edu.tw
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