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Vol. 47 No. 3, 2008
Mitochondrial DNA Diversity of Tor tambroides Valenciennes
(Cyprinidae) from Five Natural Populations in Malaysia
Yuzine
B. Esa1,2,*, Siti Shapor Siraj2, Siti Khalijah Daud2, Khairul Adha A.
Rahim2, Jeffrine Rovie Ryan Japning3, and Soon Guan Tan2
1Faculty
of Resource Science and Technology, Universiti Malaysia Sarawak, 94300
Kota Samarahan, Sarawak, Malaysia
2Biology Department, Universiti Putra Malaysia 43400
Serdang, Selangor Darul-Ehsan, Malaysia
3Institute of Biodiversity, Bukit Rengit, 28500
Lanchang, Pahang, Malaysia
Yuzine
B. Esa, Siti Shapor Siraj, Siti Khalijah Daud, Khairul Adha A. Rahim,
Jeffrine Rovie Ryan Japning, and Soon Guan Tan (2008) In this
study, we examined the genetic structure of Tor tambroides
Valenciennes, an important indigenous freshwater fish species in
Malaysia, using sequence analysis of 464 base pairs of the
mitochondrial cytochrome c oxidase I (COI) gene. In total, 92 T.
tambroides samples were analyzed from 4 locations on Peninsular
Malaysia (n = 87) and a single population from Sarawak (Batang Ai, n =
5) on Borneo I., and 4 sequences of Tor douronensis from Sarawak were
used for comparisons. In total, 9 haplotypes were found, with 7
haplotypes being unique and 2 haplotypes being shared among the 5
populations. The phylogenetic analysis using Neighbor-joining
(NJ) and maximum-parsimony (MP) methods supported the monophyletic
status between T. tambroides and T. douronensis, thus suggesting their
status as different species. The clustering of all T. tambroides
samples into a single clade suggested that their genetic identity
belongs to a single species. The sharing of haplotype HKE4
between Batang Ai of Sarawak (n = 4) and Perak of Peninsular Malaysia
(n = 3) reflects the historical connection of drainages between the
regions possibly during Pleistocene glaciation periods. Limited
variations were found among all peninsular T. tambroides
populations. The low level of mitochondrial (mt)DNA differences
currently found among T. tambroides populations is probably due to the
high proportion of the HKE1 haplotype being found in all the
populations (0.736 - 1.000), or the small number of samples used in the
present study. Overall, the present study was able to shed light
on the phylogenetic relationships and genetic structure of T.
tambroides in Malaysia.
Key words: Tor
tambroides, Freshwater fish, mtDNA COI, Population structure.
*Correspondence: Tel: 60-38-9466613. Fax:
60-38-6567454. E-mail:kelahzine@yahoo.com
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