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Vol. 47 No. 6, 2008
Genetic Structure of Steller Sea Lion (Eumetopias jubatus) Rookeries in the Sea of Okhotsk
Satoshi Koyama1,2, Shoko Fujita2, Tadao Hirota3, Toshiyuki Satoh2,*, Yoshiaki Obara2, Hiroshi Hoshino4, Akihiko Wada5, Vladimir N Burkanov6,7, and Kazuo Wada8
1United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
2Department
of Veterinary Medicine, Faculty of Agriculture, Tokyo University of
Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo 183-8509, Japan
3Department of Biology, Faculty of Science, Yamagata University, Kojirakawa-machi 1-4-2, Yamagata 990-8560, Japan
4Hokkaido-Wakkanai Fisheries Experimental Station,Wakkanai, Hokkaido 097-0001 Japan
6Kamchatka Branch of the Pacific Institute of
Geography Far East Branch, Russian Academy of Sciences, 6 Partizanskaya
St., Petropavlovsk-Kamchatsky 683000, Russia
7National Marine Fisheries Service, Alaska Fisheries
Science Center, National Marine Mammal Laboratory, 7600 Sand Point Way
NE, Seattle, WA 98115, USA
8Tounoji-Suginyama 9-18, Inuyama, Aichi, Japan
Satoshi
Koyama, Shoko Fujita, Tadao Hirota, Toshiyuki Satoh, Yoshiaki Obara,
Hiroshi Hoshino, Akihiko Wada, Vladimir N Burkanov, and Kazuo Wada
(2008) Genetic relationships among 4 Steller sea lion (Eumetopias jubatus)
rookeries in the Kuril Is. (Brat Chirpoev, Lovushki, Raykoke, and
Antsiferov Is.) and 1 at Iony I. were evaluated using an inter-simple
sequence repeat (ISSR)-polymerase chain reaction assay. An ISSR
primer yielded 15 amplification products, almost all of which were
polymorphic. An analysis of molecular variance test revealed that
94.4% of the total genetic variation was attributable to differences
among individuals within rookeries, and 5.6% was attributable to those
among rookeries (ФST = 0.056). Pairwise ФST
values were relatively high between rookeries of the Kuril Is. and that
on Iony I., while no significant genetic differentiation was detected
among rookeries of the Kuril Is. Since previous research using
mitochondrial DNA markers revealed the presence of unique haplotype(s)
on each island of the Kuril Is., our results suggest that males, rather
than females, contribute to gene flow among the islands.
Key words: Marine mammals, Steller sea lion, Local population, Genetic differentiation, Conservation.
*Correspondence: Tel: 81-423-675629. Fax: 81-423-675628. E-mail:tsatoh@cc.tuat.ac.jp
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