Vol. 48 No. 5, 2009

Enhancement of Vitellogenin Synthesis by Serotonin in the Giant Freshwater Prawn Macrobrachium rosenbergii (de Man)

Ching-Ming Kuo¹, Ying-Nan Chen², Hui-Feng Fan¹, Hsiang-Chieh Chuang¹, and Shu-Ling Hsieh^3,*

¹Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Jiawshi, Ilan 262, Taiwan
²Department of Aquaculture, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan
³Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan

Ching-Ming Kuo, Ying-Nan Chen, Hui-Feng Fan, Hsiang-Chieh Chuang, and Shu-Ling Hsieh (2009) Serotonin (5-hydroxytryptamine, 5 HT) plays important roles in regulating diverse physiological processes in crustaceans.  The stimulatory effect of 5 HT on vitellogenin (Vg) synthesis in the giant freshwater prawn Macrobrachium rosenbergii (de Man) is presented in this paper.  During a 16 d experimental period, hemolymph was collected from both intact and bilateral eyestalk-ablated prawns 2 d after the administration of 5 HT, which was periodically injected into prawns on day 2 and every 4th day thereafter.  The vitellogenin concentration in the hemolymph was quantified using an ELISA technique.  The results showed that 5 HT enhanced the process of Vg synthesis in a dose-dependent manner.  The fact that 5 HT is able to stimulate Vg synthesis in eyestalk-ablated prawns in a similar manner as in intact prawns, and that the synthesis and release of Vg from the hepatopancreas and the increment of total Vg mRNA in hepatopancreas were all enhanced by 5 HT stimulation in the presence of ganglion tissues in vitro suggest that the stimulatory action of 5 HT on Vg synthesis is mediated through a factor, likely a vitellogenesis-stimulating hormone in brain, and thoracic and abdominal ganglion tissues.

Key words: Vitellogenin, Serotonin, Ganglion, Eyestalk-ablation, Macrobrachium rosenbergii.

*Correspondence: Tel: 886-7-3617141.  Fax: 886-7-3628844.   E-mail:slhsieh@ntu.edu.tw