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Vol. 48 No. 5, 2009
Artificial Insemination and Early Embryonic Development of the Mangrove Crab Perisesarma bidens (De Haan) (Crustacea: Brachyura)
Md. Moniruzzaman Sarker1,2,*, Md. Sirajul Islam2, and Tsuyoshi Uehara2
1Department of Zoology, University of Rajshahi, Rajshahi- 6205, Bangladesh
2Laboratory
of Developmental Biology, Department of Chemistry, Biology and Marine
Science, Faculty of Science, University of the Ryukyus, 1 Senbaru,
Nishihara City, Okinawa 903-0213, Japan
Md. Moniruzzaman Sarker, Md. Sirajul Islam, and Tsuyoshi Uehara (2009) The
aim of this study was to explore a technique of artificial insemination
and document the early embryonic development of the mangrove sesarmid
crab Perisesarma bidens (De
Haan). Observations were made on fertilization of the eggs of
this crab by artificial insemination up to hatching under laboratory
conditions. The female extruded the eggs into the abdominal
cavity 24-48 h after copulation. Unfertilized eggs were collected
from the pleopods of the female immediately after laying and were
stored in 80% filtered sea water (FSW). Sperm were removed from
the spermatheca of the same female and diluted in 80% FSW. The
unfertilized eggs and sperm were shaken well in a glass beaker for
artificial insemination. The eggs were rinsed 3-5 times with 80%
FSW after 5 min of mixing of sperm with ova. Eggs were incubated
in 100 ml flat cylindrical culture bottles containing 70 ml of 80% FSW
at 25°C in a water bath. The fertilization membrane was observed
3-5 min after insemination. The other 2 outerlayers of fertilized
eggs were also observed 8-10 min after insemination. The internal
yolk of the egg is the major source of nutrition for developing
embryos. An average of 65% of fertilized eggs hatched as 1st
zoeae after 17 d of incubation. It is therefore feasible to
produce embryos by artificial insemination during the peak breeding
season from May to Sept. This is the first report of early
embryonicdevelopment from fertilization to larval hatching of P. bidens by artificial insemination.
Key words: Perisesarma bidens, Artificial insemination, Cleavage, Embryonic development, Zoeae.
*Correspondence: Tel: 880-721-750041 ext. 4119. Fax: 880-721-750064. E-mail:sarkerphd@yahoo.com
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