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Vol. 50 No. 6, 2011
Intrageneric Karyotypic Variation in Pseudopaludicola (Anura: Leiuperidae) and Its Taxonomic Relatedness
Eduardo R. Fávero1, Ana C. P. Veiga-Menoncello1, Denise C. Rossa-Feres2, Christine Strüssmann3, Ariovaldo A. Giaretta4, Gilda V. Andrade5, Patrick Colombo6, and Shirlei M. Recco-Pimentel1,*
1Departamento
de Anatomia, Biologia Celular e Fisiologia e Biofísica, Instituto de
Biologia, Univ. Estadual de Campinas (UNICAMP), Campinas 13083-863, São
Paulo, Brazil
2Departamento de Zoologia e
Botânica, Instituto de Biociências, Letras e Ciências Exatas, Univ.
Estadual Paulista (UNESP), Campus de São José do Rio Preto, São José do
Rio Preto 15054-000, São Paulo, Brazil
3Departamento
de Ciências Básicas e Produção Animal, Faculdade de Agronomia e
Medicina Veterinária, Univ. Federal de Mato Grosso (UFMT), Cuiabá
78060-900, Mato Grosso, Brazil
4Faculdade de Ciências Integrades do Pontal, Univ. Federal de Uberlândia (UFU), 384302-000, Ituiutaba, MG, Brazil
5Departamento de Biologia, Centro de Ciências da Saúde, Univ. Federal do Maranhão (UFMA) São Luis, 65085-580, Maranhão, Brazil
6Museu de Ciências Naturais da Fundação Zoobotânica do Rio Grande do Sul Porto Alegre, 90690-000, Rio Grande do Sul, Brazil
Eduardo R. Fávero, Ana C. P. Veiga-Menoncello, Denise C.
Rossa-Feres, Christine Strüssmann, Ariovaldo A. Giaretta, Gilda V.
Andrade, Patrick Colombo, and Shirlei M. Recco-Pimentel (2011) Herein, we report karyological data for Pseudopaludicola
frogs, with the main objective to investigate the confusing taxonomic
identification of these anurans. The samples analyzed included
topotypes of P. falcipes and P. ameghini (currently considered a synonym of P. mystacalis) in addition to specimens of P. mystacalis and P. ternetzi and of 8 additional populations of Pseudopaludicola
from several regions of Brazil. Interspecific variations in the
chromosome number, location of the nucleolar organizer region (NOR),
and banding patterns were observed. The karyotype of P. falcipes consisted of 2n = 22 chromosomes, whereas P. ameghini (sensu Cope 1887) and P. ternetzi had 2n = 20 and P. mystacalis had 2n = 16 chromosomes. The chromosome number 2n = 16 was also found in specimens of populations from Santa Fé do Sul, Vitória Brazil, Icém, Palestina
(southeastern Brazil), Barreirinhas, Urbano Santos (northeastern
Brazil), Poconé, and Santa Terezinha (west-central Brazil).
Nevertheless, in some individuals from Icém and Poconé, we found 2n = 20 and 2n
= 22 chromosomes, respectively. This variation in chromosome number
indicates diverse taxa coexisting in sympatry. The karyotypic patterns
of 2n = 20 in P. ameghini (sensu Cope 1887) and 2n = 16 in P. mystacalis are clearly indicative of 2 distinct taxonomic units. Therefore, our data corroborate the removal of P. ameghini from synonymy with P. mystacalis as previously suggested. We define the karyotype of P. falcipes as 2n = 22 and of P. ternetzi as 2n = 20 and reinforce the need for a taxonomic revision of this genus.
Key words: Pseudopaludicola, Cytotaxonomy, Karyotype, Ag-NOR, C-band.
*Correspondence: Tel: 55-19-35216128. Fax: 55-19-35216185. E-mail:shirlei@unicamp.br
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