Vol. 51 No. 1, 2012
Molecular and Morphological Investigations of Shovel-Nosed
Lobsters Thenus spp.
(Crustacea: Decapoda: Scyllaridae) in Thailand
Iamsuwansuk1, Jessada Denduangboripant1,*, and Peter J.F. Davie2
of Biology, Faculty of Science, Chulalongkorn Univ., Phayathai Road,
Phatumwan, Bangkok 10330 Thailand
2Queensland Museum, PO Box 3300, South Brisbane,
Queensland 4101, Australia
Apinan Iamsuwansuk, Jessada Denduangboripant, and
Peter J.F. Davie (2012) Shovel-nosed lobsters (Thenus spp.) (or kang-kradan in Thai) are the basis
of an increasingly important fishery in Thailand and other tropical
Indo-West Pacific countries. In the past, only a single species was
recognized, Thenus orientalis. However, a recent
taxonomic revision, using both morphological and DNA sequence analyses,
established that at least 3 species occur in Thai waters. The present
work was designed to test the results of that earlier study as applied
to the Thai fishery, and extend it by using a broader sampling regime.
Thirty adult Thenus specimens
were sampled from 3 provinces: Chonburi (on the eastern Gulf of
Thailand), Phetchaburi (on the western Gulf of Thailand), and Phuket
(on the Andaman Sea). Genomic DNA was extracted from pereiopods, and
the mitochondrial cytochrome c oxidase subunit 1 (COI) gene was
amplified and sequenced. A 403-base pair nucleotide data matrix was
used to derive phylogenetic trees using maximum parsimony. The
molecular phylogeny clearly separated the Thai specimens into 3 clades:
13 individuals of T. indicus,
7 of T. orientalis, and 10 of
T. unimaculatus. The
recently proposed morphological criteria were largely effective in
separating Thenus species;
however some of the morphometric ratios given in the previous paper
need to be adjusted. New local Thai names are proposed: kang-kradan
thammada (common shovel-nosed lobster) for T. indicus, kang-kradan kha-lai (spotted-leg
shovel-nosed lobster) for T.
orientalis, and kang-kradan
kha-muang (purple-leg shovel-nosed lobster) for T. unimaculatus. It is evident that
there is some ecological separation of the different species, and we
hope this increased knowledge can be used to help establish sustainable
management of their exploitation in Thailand.
Key words: Cytochrome c oxidase subunit I, Molecular
phylogenetics, Shovel-nosed lobster, Thailand, Thenus.
*Correspondence: Tel: 66-22185378. Fax: 66-2-2185386.