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Vol. 51 No. 8, 2012
Respiratory Burst Activity in Head Kidney and Spleen Leukocytes of Tilapia (Oreochromis mossambicus) under Acute Osmotic Stress
Bharath Kumar Velmurugan1, Ing-Fong Jiang1, Hung-Yuan Shih1, Der-Nan Lee2, and Ching-Feng Weng1,*
1Institute of Biotechnology, National Dong Hwa Univ., Hualien 974, Taiwan
2Department of Animal Science, National Ilan Univ., 1 Shen-Lung Rd., Sec. 1, Ilan 260, Taiwan
Bharath Kumar-Velmurugan, Ing-Fong Jiang, Hung-Yuan Shih, Der-Nan Lee, and Ching-Feng Weng (2012) Tilapia Oreochromis mossambicus
is capable of surviving in a wide range of salinities and
temperatures. In the present study, we determined how salinity
influences the respiratory burst (RB) activity of tilapia. At
first, tilapia were exposed to freshwater (FW) or 25 ppt seawater (SW),
and serum was isolated from these groups. Further, leukocytes of
the head kidneys (HKs) and spleen were isolated from tilapia exposed to
300 or 500 mOsm osmolality. Following osmolality exposure, cells
were incubated (1) without serum (as the control), (2) with serum of
tilapia exposed to fresh water (FW), and (3) with serum of tilapia
exposed 25 ppt seawater (SW). A cell viability assay indicated
that exposure of HK and spleen leukocytes to 300 and 500 mOsm up to 24
h failed to inhibit cell viability. When comparing RB activities
at the 2 osmolalities without adding serum, HK and spleen leukocytes
cultured at 500 mOsm osmolality for 4, 8, and 24 h were found to have
higher activities than those cultured at 300 mOsm osmolality. RB
activities of HK and spleen leukocytes showed significant decreases in
both 300 and 500 mOsm incubated cells after adding serum, and the
decrease was more distinct at an osmolality of 500 mOsm. These
results imply that the addition of serum might cause a suppressive
effect on cellular parameters rather than improving the effects of
cytokines on cellular parameters.
Key words: Respiratory burst activity, Stress-activated serum, Freshwater (FW)-exposed tilapia, Hyperosmolality.
*Correspondence: Tel: 886-3-8633637. Fax: 886-3-8630255. E-mail:cfweng@mail.ndhu.edu.tw
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