Vol. 51 No. 8, 2012
Inheritance of Microsatellite Loci and Their Application for
Pedigree Analysis of the Polyploid Persian Sturgeon Acipenser persicus (Acipenseridae)
Mehdi
Moghim1,2,3,*, Soon Guan Tan3, Arash Javanmard4,
Mohamad Pourkazemi5, and Jothi Malar Panandam6
1Department
of Genetics, Caspian Sea Ecology Research Center, PO Box 961, Sari, Iran
2Department of Stock Assessment , Caspian Sea Ecology
Research Center, PO Box 961, Sari 48471-53948, Iran
3Department of Cell and Molecular Biology, Faculty of
Biotechnology and Biomolecular Sciences, University Putra Malaysia,
43400 UPM, Serdang, Selangor, Malaysia. E-mail:sgtan_98@yahoo.com
4Department of Genomics, Agricultural Biotechnology
Research Institute of Iran (ABRII), PO Box 31535-1897, Mahdasht Road,
Karaj, Iran. E-mail:Javanmard@abrii.ac.ir
5Department of Genetics, International Sturgeon
Research Institute, PO Box 41635-3464, Rasht, Iran.
E-mail:pkazemi_m@yahoo.com
6Department of Animal Science, Faculty of Agriculture,
University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
E-mail:Jothi@agri.upm.edu.my
Mehdi Moghim, Soon Guan Tan, Arash
Javanmard, Mohamad Pourkazemi, and Jothi Malar Panandam (2012) Identification
of suitable molecular markers for correctly assigning progenies to
their parents particularly during the early stages of development is
vital for aquaculture breeding programs. Developing such markers in
sturgeons, that have a polyploid ancestry, is particularly challenging
because many markers exhibit polysomic inheritance. In the present
study, 2 F1 families of 23 and 28 larvae were produced in 1
× 1 crosses of Persian sturgeon. Eleven microsatellite loci were used
to genotype the parents and their offspring. An analysis of inheritance
patterns demonstrated classical mendelian disomic inheritance in all
but 2 of the markers tested in the offspring. Two loci exhibited
inheritance patterns consistent with a parental null allele that was
consistently inherited by approximately 1/2 of the offspring screened.
Thus, the markers tested here can be used for parental assignment
testing and for population genetic studies. They can also be used as
reliable molecular markers for constructing a genetic linkage map for
the target species that will be essential for future quantitative trait
locus mapping purposes. This is the 1st report of mendelian segregation
testing in Persian sturgeon for cross-species amplification of
single-locus DNA microsatellite markers.
Key words: Microsatellite loci, Persian
sturgeon, Family studies, Mendelian inheritance, Caspian Sea.
*Correspondence: E-mail:mehdi.moghim@gmail.com;
Moghim_m@yahoo.com
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