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Vol. 52, 2013
Comparative proteomics analysis of normal and
memory-deficient Drosophila melanogaster heads
Tian-Ren
Lee†, Hsiao-Yun Lee†,
Shun-Hong Huang, Hsin-Tzu Chan, Ping-Chiang Lyu and Hong-Lin Chan*
Institute
of Bioinformatics and Structural Biology, and Department of Medical
Sciences, National Tsing Hua University, 101 Kuang-Fu Road, Section 2,
Hsinchu 30013, Taiwan
Abstract
Background: Learning and memory are
extremely complex and dynamic processes. Proteins that participate in
memory formation are strictly regulated by various pathways and may
require protein synthesis and/or posttranslational modifications. To
examine the formation of memory, Drosophila
was genetically engineered with the mutated memory-related gene, AmnX8, which induces
normal learning and memory behavior within the first 30 min of
training. However, the process through which learning occurred could
not be retained after the 30 min of training, indicating that these
mutants possessed deficits in middle-term memory. A proteomics platform
based on two-dimensional differential gel electrophoresis and
matrix-assisted laser desorption/ionization time of flight mass
spectrometry was employed to examine the head proteome alterations
between the wild-type 2u strain and the memory-deficient mutant AmnX8 strain.
Results: The results indicated that 30
differentially expressed head proteins that mainly function in
metabolic pathways and cell structure/cytoskeleton proteins were
involved in memory formation. A bioinformatics analysis demonstrated
that mitochondrial proteins had critical roles in modulating this
process.
Conclusions: This is the first
study of a comparative head proteomics analysis of a memory mutant
strain and a normal control fruit fly strain. The fundamental
proteomics analysis provides potential candidates for further
elucidation of the biological mechanism of the memory formation process
in Drosophila.
Key words: Drosophila; Brain; Proteomics;
2D-DIGE; Memory.
*Correspondence: E-mail: hlchan@life.nthu.edu.tw †Equal
contributors.
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