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Vol. 52, 2013
Expressions of oxidative stress-related genes and antioxidant
enzyme activities in Mytilus galloprovincialis (Bivalvia,
Mollusca) exposed to hypoxia
Seonock
Woo1, Vianney Denis2, Hyokyung Won1,
Kyungsoon Shin3, Gunsup Lee1, Taek-Kyun Lee1
and Seungshic Yum1*
1South
Sea Environment Research Department, Korea Institute of Ocean Science
and Technology, Geoje 656-834, Republic of Korea
2DBiodiversity Research Center, Academia Sinica,
Taipei 11529, Taiwan
3Ballast Water Center, Korea Institute of Ocean
Science and Technology, Geoje 656-834, Republic of Korea
Abstract
Background: In this study, we
investigated transcription and enzyme level responses of mussels Mytilus galloprovincialis exposed to
hypoxic conditions. Genes for catalase (CAT), cytochrome P450,
glutathione Stransferase (GST), metallothionein, superoxide dismutase
(SOD), cytochrome c oxidase subunit 1 (COX-1), and NADH dehydrogenase
subunit 2 were selected for study. Transcriptional changes were
investigated in mussels exposed to hypoxia for 24 and 48 h and were
compared to changes in control mussels maintained at normal oxygen
levels. Activities of CAT, GST, and SOD enzymes, and lipid peroxidation
(LPO) were also investigated in mussels following exposure to hypoxia
for 24, 48, and 72 h.
Results: Relative to the control group, the CAT
activity decreased in all hypoxia treatments, while the activity of GST
significantly increased in mussels exposed to hypoxia for 24 and 48 h,
but decreased in those exposed for 72 h. The LPO levels were
significantly higher in mussels in the 24- and 48-h hypoxia treatments
than those in the control mussels, but there was no significant change
in the SOD activities among all hypoxia treatments. Messenger RNA
levels for the CAT, cytochrome P450, GST, metallothionein, and SOD
genes were not significantly affected by hypoxic conditions for 48 h,
but the expressions of the COX-1 and NADH dehydrogenase subunit 2 genes
were significantly repressed in mussels in both the 24- and 48-h
exposure treatments.
Conclusions: These results
demonstrate the transcriptional stability and changes among several
genes related to oxidative stress under oxygen-depletion conditions in M. galloprovincialis and provide
useful information about the modulation of antioxidant enzyme
activities induced by hypoxia in a marine animal.
Key words: Mytilus galloprovincialis; Hypoxia;
Oxidative stress-related gene; Antioxidant enzymes.
*Correspondence: E-mail: syum@kiost.ac
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