Vol. 59, 2020

(update: 2020.10.21; 11.30)
 

Seasonality and Longevity of the Functional Chloroplasts Retained by the Sacoglossan Sea Slug Plakobranchus ocellatus van Hasselt, 1824 Inhabiting A Subtropical Back Reef Off Okinawa-jima Island, Japan

Shu Chihara, Takashi Nakamura, and Euichi Hirose* 

Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan. *Correspondence: E-mail: euichi@sci.u-ryukyu.ac.jp (Hirose)
E-mail: ringoamemh@icloud.com (Chihara); takasuke@sci.u-ryukyu.ac.jp (Nakamura)

Received 18 July 2020 / Accepted 12 October 2020
Communicated by Yoko Nozawa

Plakobranchus ocellatus is a sacoglossan sea slug that feeds on multiple algal species and retains chloroplasts as kleptoplasts for several months. The seasonal differences in the photosynthetic properties of kleptoplasts were examined in sacoglossans collected from a subtropical back reef off of Okinawa-jima (26°21'55"N 127°44'10"E) in 2017–2018. The effective quantum yield of photosystem II in kleptoplasts indicated that stronger ambient light causes more stress in kleptoplasts. The maximum quantum yields (QY) at 20°C, 30°C, and 40°C indicated that kleptoplasts were more functional in photosynthesis in winter than in spring or summer, whereas kleptoplasts may have the highest tolerance to high temperatures in summer. In the long-starvation experiment (LSE), the relative ratio of body weight (relW) linearly decreased and the sacoglossans died within 2 months in the total dark condition, whereas in the LSE with illumination, the animals survived up to 5 months. The time course for the decrease in the relative ratio of the QY (relQY) in the LSE indicated that the photosynthetic function was almost normal for 2
months, regardless of the presence or absence of illumination, after which time relQY gradually decreased to zero. In the field, P. ocellatus continuously took up new kleptoplasts that have suitable properties of photosynthetic ability for each season. In a subtropical environment, in which water temperatures vary from below 20°C to above 30°C, seasonal changes could cause a temporary shortage of algal food and affect the photosynthetic activity of P. ocellatus kleptoplast. Our results, however, indicated the kleptoplasts of P. ocellatus functioned normally for several months and maintained the presence of this sacoglossan in a subtropical environment throughout the year.

Key words: Kleptoplasty, Long-starvation experiment, Long-term retention, Pulse-amplitude-modulated fluorescence, Total darkness.

Citation: Chihara S, Nakamura T, Hirose E. 2020. Seasonality and longevity of the functional chloroplasts retained by the sacoglossan sea slug Plakobranchus ocellatus van Hasselt, 1824 inhabiting a subtropical back reef off Okinawa-jima Island, Japan. Zool Stud 59:65. doi:10.6620/ZS.2020.59-65.