Vol. 60, 2021
Seasonal Growth of the Purple Sea Urchin Heliocidaris crassispina Revealed by Sequential Fluorochrome Tagging
Juan Diego Urriago Suarez1,2,3, Jane C.Y. Wong2, Gilbert Lui4, Clement P. Dumont5, Jian-Wen Qiu2,3, and Monthon Ganmanee1,*
of Animal Production Technology and Fisheries, Faculty of Agricultural
Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok
10520, Thailand. *Correspondence: E-mail: email@example.com
(Ganmanee). Tel: +6623298140.
E-mail: firstname.lastname@example.org (Suarez)
2Department of Biology, Hong Kong Baptist University, Hong Kong, China. E-mail: email@example.com (Wong)
3Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), China. E-mail: firstname.lastname@example.org (Qiu)
4Department of Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China. E-mail: email@example.com (Lui)
5School of Biological Sciences, The University of Hong Kong, Hong Kong, China. E-mail: firstname.lastname@example.org (Dumont)
Received 8 January 2020 / Accepted 6 May 2021
Communicated by Chih-Ming Hung
studies have applied fluorochrome tagging to examine the growth of
animals with calcified skeletons, but most of them have used a single
tag to determine the annual growth rate. We used sequential
fluorochrome tagging to study the seasonal growth of the purple sea
urchin Heliocidaris crassispina
in Hong Kong waters from February 2012 to February 2013. Sea urchins
ranging from 18.9 to 42.7 mm in test diameter had a yearly growth from
0.6 to 13.0 mm. During that year, the sea urchins grew from 0.6 to 5.0
mm in test diameter during the first six months, and from 0.4 to 10.2
mm in test diameter in the second six months. The seasonal differences
in growth were confirmed using the von Bertalanffy model. The growth
was obvious for young sea urchins, especially for individuals less than
5 years old, but was not evident for sea urchins older than 7 years.
The seasonal differences in growth were probably related to the
reproductive cycle and the seasonal differences in environmental
conditions. Our empirical results provided the first evidence of
seasonal changes in growth for H. crassispina,
demonstrating the usefulness of sequential fluorochrome tagging in
studying the growth of sea urchins in the field. We also discovered the
problem of low recovery of tagged individuals and provided
recommendations to improve the tagging procedure.
Key words: Calcein, Calcein blue, Fluorochrome, Seasonal growth model, Sea urchin, Tagging.
Citation: Suarez JDU, Wong JCY, Lui G, Dumont CP, Qiu JW, Ganmanee M. 2020. Seasonal growth of the purple sea urchin Heliocidaris crassispina revealed by sequential fluorochrome tagging. Zool Stud 60:0mm. doi:-.
S2 | DataSet S1