Vol. 60, 2021
Moderate Population Structure
in Drosophila sturtevanti
from the South American Atlantic Forest Biome
Memari Trava1, Rogério
Pincela Mateus2, Luciana
Paes de Barros Machado2,
and Lilian Madi-Ravazzi1,*
de Biociências, Letras e Ciências Exatas, Universidade Estadual
Paulista “Júlio de Mesquita Filho” (UNESP), Cristóvão Colombo Street,
2265 – Jardim Nazareth, São José do Rio Preto, SP 15054-000, Brazil.
*Correspondence: E-mail: firstname.lastname@example.org (Madi-Ravazzi). Tel: +55
17 3221-2389. Fax: +55 17 3221-2390.
E-mail: email@example.com (Trava)
2Universidade Estadual do Centro-Oeste (UNICENTRO),
Campus CEDETEG, Élio Antonio Dalla Vecchia Alley, 838, Vila Carli,
Guarapuava, PR 85040-167, Brazil. E-mail: firstname.lastname@example.org
(Mateus); email@example.com (Machado)
Received 30 May 2020 / Accepted 25 May
Communicated by Chung-Pin Lin
Drosophila sturtevanti is a widely
distributed Neotropical species. In South America, it is abundant and
adapted to different phytophysiognomies of the Atlantic Forest biome.
Reproductive, chromosomal and enzymatic studies have indicated the
existence of a differentiation among D.
sturtevanti populations. In this work, the level of genetic
diversity and the population genetic structure, using four population
groupings, were analyzed. One hundred and twenty-six D. sturtevanti males collected from
nine forest fragments were analyzed for 11 species-specific
microsatellite loci. A total of 109 alleles, ranging from 2 to 16
alleles per locus, were detected. The highest mean observed
heterozygosity - HO
was estimated in samples from the largest collection areas, and the
lowest HO was from
a population where fire events are common. A low molecular variation,
around 3% among populations and negative among groups, an absence of
genetic and geographic correlations and a moderate genetic
differentiation - FST
= 0.0663 - indicated that D.
sturtevanti is not strongly structured. Besides no overall
genetic and geographic distance correlation, the pair of closest
geographically populations Matão and Nova Granada showed the lower
differentiation through FST,
DC and Neighbor Joining tree. Ribeirão da Ilha - RDI, an isolated
insular population, was the most differentiated according to FST, DC and a
cluster-based Bayesian analysis. The isolation of RDI that resulted in
significant divergence could be ancient, because of sea level
regressions/transgressions, or more recently via founder effect/genetic
drift by anthropic action carrying D.sturtevanti
hosts from continent to island. This work was important to understand
the genetic variability distribution of a Neotropical forest-dwelling Drosophila species, using for the
first time, a wide population distribution approach.
Key words: Saltans group,
Forest fragmentation, Genetic diversity, Simple sequence repeats,
Citation: Trava BM, Mateus RP, de Barros
Machado LP, Madi-Ravazzi L. 2021. 2021. Moderate population structure
in Drosophila sturtevanti
from the South American Atlantic Forest biome. Zool Stud 60:46.