Vol. 60, 2021
Molecular Identification of Mid
to Final Stage Slipper Lobster Phyllosoma Larvae of the Genus Chelarctus (Crustacea: Decapoda:
Scyllaridae) Collected in the Pacific with Descriptions of Their Larval
Kenta Ueda1,2, Takashi Yanagimoto3, Seinen Chow3,*, Mari Kuroki1, and Takashi Yamakawa1,*
of Aquatic Bioscience, Graduate School of Agricultural and Life
Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo, Tokyo 113-8657,
Japan. *Correspondence: E-mail: firstname.lastname@example.org (Yamakawa)
E-mail: email@example.com (Ueda); firstname.lastname@example.org (Kuroki)
2Fisheries Agency, Kasumigaseki 1-2-1, Chiyoda, Tokyo
100-8907, Japan. E-mail: email@example.com (Ueda)
3Fisheries Resources Institute, Fukuura 2-12-4,
Kanazawa, Yokohama, Kanagawa 236-8648, Japan. *Correspondence: E-mail:
E-mail: firstname.lastname@example.org (Yanagimoto)
Received 24 May 2021 / Accepted 11
Communicated by Benny K.K. Chan
Morphological descriptions of
phyllosoma larvae are essential for correct species identification and
investigating the spatiotemporal distribution and recruitment process
of spiny and slipper lobsters. Species identification of the phyllosoma
larvae in the Scyllarinae subfamily is particularly difficult because
of the morphological similarities among species and the scarcity of
morphological information describing correct species identity. We
extracted mid- to final-stage (V to VIII) phyllosoma larvae (n = 12) belonging to the subfamily
Scyllarinae from several plankton samples collected in the Pacific and
then performed molecular species identification using mitochondrial DNA
COI and 16S rDNA
sequence analyses. Three larvae collected around the Ryukyu Archipelago
were identified as Chelarctus aureus
(stage VI to VIII), and four collected around the Ryukyu Archipelago
and Ogasawara Islands were identified as C. virgosus (V to VIII). One larva
(V) collected in the central South Pacific was determined to be a
subspecies of C. crosnieri.
DNA barcodes could not be made for the remaining four larvae (V to
VIII) collected around the Ryukyu Archipelago (designated by? Chelarctus sp-1). Based on the
morphological characteristics of the C.
virgosus phyllosoma described in this study and the adult
distributions reported to date, C.
cultrifer phyllosomas previously reported in Japanese and
Taiwanese waters are likely to be C.
virgosus. This paper also presents a set of diagnostic
morphological characteristics that can be used to discriminate among
these four species of Chelarctus
and from other genera in the subfamily Scyllarinae.
Key words: Phyllosoma larvae,
Slipper lobster, Molecular analysis, Morphology, Identification, Chelarctus.
Citation: Ueda K, Yanagimoto T, Chow S,
Kuroki M, Yamakawa T. 2021. Molecular identification of mid to final
stage slipper lobster phyllosoma larvae of the genus Chelarctus (Crustacea: Decapoda:
Scyllaridae) collected in the Pacific with descriptions of their larval
morphology. Zool Stud 60:75.