Zoological Studies

Vol. 61, 2022

(update: 2022.9.21)

Finding the Missing Puzzle Piece of the Nisto Stage in the Larval Cycle of the Slipper Lobster Scyllarides squammosus: A Molecular and Morphological Approach

Chiho Hidaka1, Chien-Hui Yang2, and Kaori Wakabayashi3,*

1School of Applied Biological Science, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima, Hiroshima 739-8528, Japan. E-mail: chihonman67@gmail.com (Hidaka)
2Institute of Marine Biology and Centre of Excellence for the Oceans, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 202301, Taiwan. E-mail: chyang@mail.ntou.edu.tw (Yang)
3Graduate School of Integrated Sciences for Life, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima, Hiroshima 739-8528, Japan. E-mail: kaoriw@hiroshima-u.ac.jp (Wakabayashi)

Received 18 June 2021 / Accepted 2 September 2022
Communicated by Benny K.K. Chan

Slipper and spiny lobsters are crustaceans that are in high demand and possess great commercial potential as a valuable food. Their early life stages are important for understanding their distribution in addition to lobster resource ecology; however, minimal information about slipper lobsters compared to spiny lobsters is available. Biological information concerning the transition stage from the planktonic to the benthic phase, the so-called nisto stage, is limited probably due to the short duration of this stage. An individual scyllarid nisto was discovered while scuba diving off Chichijima Island. DNA analyses using mitochondrial 16S rRNA and cytochrome c oxidase subunit 1 (COI) genes confirmed this specimen to be Scyllarides squammosus (H. Milne Edwards, 1837). Detailed morphological observation of this specimen and its comparison with previous reports on Scyllarides nistos suggest that the diagnostic character of S. squammosus nisto is the pleura of the second to fifth pleonites possessing prominent teeth entirely on the lateral margin. Other morphological characteristics are the carapace with the widest distance in the middle and the second to fifth pleonites bearing two tubercles on each side. This report describes identification of the first worldwide record of a Scyllarides nisto that was confirmed by molecular barcoding.

Key words: Decapoda, Taxonomy, Larva, Settlement, DNA barcoding.

Citation: Hidaka C, Yang CH, Wakabayashi K. 2022. Finding the missing puzzle piece of the nisto stage in the larval cycle of the slipper lobster Scyllarides squammosus: a molecular and morphological approach. Zool Stud 61:73.

Supplementary materials: Table S1