Zoological Studies

Vol. 61, 2022

(update: 2022.11.2)

Identification and Distribution of Wedge Clams (Donacidae: Bivalvia) in Thailand by Geometric Morphometric and Molecular Analysis

Cheewarat Printrakoon1,*, Sasimanas Unajak2, and Javier H. Signorelli1

1Animal Systematics and Ecology Specialty Unit (ASESRU), Department of Zoology, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan Road, Chatuchak, Bangkok, Thailand 10900. *Correspondence: E-mail: cheewarat.p@ku.th, fscicrp@ku.ac.th (Printrakoon)
2Department of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan Road, Chatuchak, Bangkok, Thailand 10900. E-mail: fscissmn@ku.ac.th (Unajak)
3Instituto de Biología de Organismos Marinos, IBIOMAR (CCT CONICET-CENPAT), Bvd. Brown 2915, U9120ACD Puerto Madryn, Chubut, Argentina. E-mail: jsignorelli@cenpat-conicet.gob.ar (Signorelli)

Received 16 November 2021 / Accepted 7 Oetober 2022
Communicated by Benny K.K. Chan

The phylogenetic relationship of living Thai Donacidae was herein studied. Two methodologies: geometric morphometrics (GM) and genetic analysis of COI sequence were combined and applied to identify the valid taxa and explain biodiversity and distribution pattern in this family. A total of 587 living specimen were tested to analyze the shape and size patterns by Elliptic Fourier Analysis (EFA). Shell identification and GenBank sequences were added to construct the phylogenetic relationship and haplotype network. Centroid size was useful identified to subgenus levels. Donax (Hecuba) scortum, was easily distinguished from other species by Principal Component analysis (PCA) of shell size and shape. Donax (Dentilatona) incarnatus and Donax (Deltachion) semisulcatus semisulcatus were useful identified out from Canonical Variates Analysis (CVA). Pairwise comparision of EFA was usefull in species level recognition, particularly shape overlap was observed for medium and small shell size. Based of genetic distance and and haplotype network of COI sequence Donax (Latona) faba and D. (Latona) solidus are grouped in the same clade. Intraspecific and interspecific genetic data variation of some common species in different geographical of Thailand localities was observed. Three distribution patterns of Donax species were observed along the two-marine system of Thailand.

Key words: Thailand, Bivalve, Donax, Elliptic Fourier shape analysis, COI.

Citation: Printrakoon C, Unajak S, Signorelli JH. 2022. Identification and distribution of wedge clams (Donacidae: Bivalvia) in Thailand by geometric morphometric and molecular analysis. Zool Stud 61:82.