Vol. 62, 2023
Genetic Assessment of Fertile
F1 Hybrids between Two Hercules Beetles, Dynastes maya Hardy and D. grantii Horn (Scarabaeidae)
Huang1,*, Wei-Yun Chen1,
and My Hanh Le1
Research Center, Academia Sinica, Nangang 11529, Taipei, Taiwan.
*Correspondence: E-mail: firstname.lastname@example.org (Huang).
E-mail: Weiyun33@gmail.com (Chen); Lehanh5664@gmail.com (Le)
(Received 25 July 2022 / 4 January
Accepted 2023 / Published -- 2023)
Communicated by John Wang
Hybridization, although the process
may complicate taxonomic practices, can be common between animal
species. Animal hybridization not only can help with generating
phenotypic and species diversity in nature, but also with understanding
the genetic and genomic bases of phenotypic evolution in the
laboratory. We assessed the genetic composition of captive bred F1
hybrids between two Hercules beetle species using mitochondrial CO1 and nuclear loci from a
double-digest restriction-site associated DNA sequencing (ddRADseq)
library. We showed that the F1 hybrids were genetically clustered with
samples from the maternal species, D.
grantii, based on CO1
data. Nuclear genome data, on the other hand, clearly showed that the
F1 individuals were genetically intermediate between D. maya, the paternal species, and D. grantii, based on a principle
component analysis. Our results also revealed that sampling design may
have a major impact on the inferred genetic structure and hybrid
individuals using ddRADseq data sets. We discuss the importance and
potential from studying the genomics of this hybrid progeny in terms of
understanding the origin and maintenance of both intraspecific and
interspecific phenotypic divergence and convergence.
Key words: CO1, Dynastes, Hybridization, Hybrid F1,
Citation: Huang JP, Chen WY, Le MH. 2023.
Genetic assessment of fertile F1 hybrids between two Hercules beetles, Dynastes maya Hardy and D. grantii Horn (Scarabaeidae).
Zool Stud 62:13.
materials: SM. 1丨
SM. 2丨SM. 3丨SM.4