|
Vol. 49 No. 3, 2010
Presence or Absence of the Cl− Channel Phospholemman in the Rectal Gland of Sharks: A Comparative Study
Feico M.A.H. Schuurmans Stekhoven1,*, Sjoerd E. Wendelaar Bonga1, Tsung-Han Lee2, and Andrew R. Bottrill3
1Department
of Animal Ecology and Ecophysiology, Faculty of Science, Radboud
University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The
Netherlands
2Department of Life Sciences, National Chung-Hsing University, Taichung 402, Taiwan. E-mail:thlee@dragon.nchu.edu.tw
3Protein
and Nucleic Acid Chemistry Laboratory, Proteomics Facility, University
of Leicester, Lancaster Road, Leicester, LE1 9HN, UK
E-mail:arb29@leicester.ac.uk
Feico M.A.H. Schuurmans Stekhoven, Sjoerd E. Wendelaar Bonga, Tsung-Han Lee, and Andrew R. Bottrill (2010)
In this study on the presence or absence of phospholemman expression in
the rectal glands of different orders of sharks, we observed the
following. In the clade comprised of members of the Lamniformes
and Squaliformes, the channel exists, but tends to change its primary
structure, and also appears to change to a form with a higher molecular
weight that, according to literature data, may be located in the
cytoskeleton. In the shark orders of Orectolobiformes and
Carcharhiniformes, the channel gradually disappears with Triakis scyllium
as one of the last representatives containing a largely modified
N-terminus. The final members of this clade (Carcharhinidae and
Sphyrnidae) lack the channel. From these data it was concluded
that the presence or absence of phospholemman is clade and family
dependent, and these clades have experienced different genetic
histories.
Key words: Phospholemman, Rectal gland, Shark evolution.
*Correspondence: E-mail:F.Stekhoven@science.ru.nl, Sjoerd.Wendelaar@science.ru.nl
|
