Zoological Studies

Vol. 62, 2023

(update: 2023.9.22)

Mitophylogeny of Pangasiid Catfishes and its Taxonomic Implications for Pangasiidae and the Suborder Siluroidei

Thuy Yen Duong1, Linh Thi Khanh Pham2,3, Xuyen Thi Kim Le2,3, Ngoc Tran Thi Nguyen1, Siti Azizah Mohd Nor4, and Thanh Hoa Le2,3,*

1College of Aquaculture and Fisheries, Can Tho University, 3/2 street, Can Tho City, Vietnam. E-mail: thuyyen@ctu.edu.vn (Duong)
2Immunology Department, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST). 18. Hoang Quoc Viet Rd., Cau Giay, Hanoi, Vietnam. *Correspondence: E-mail: imibtvn@gmail.com (Le)
E-mail: ntntran@ctu.edu.vn (Pham); lethikimxuyen71@gmail.com (Kim Le)
3Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
4Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. E-mail: s.azizah@umt.edu.my (Nor)

(Received 2 May 2022 / Accepted 24 July 2023 / Published 22 September 2023)
Communicated by Ka Hou Chu

Pangasiidae (catfish order: Siluriformes) comprises 30 valid catfish species in four genera: Pangasius, Pangasianodon, Helicophagus, and Pseudolais. Their systematics is frequently revised due to the addition of newly described species. Although Pangasiidae is known to be a monophyletic family, the generic and phylogenetic relationships among the taxa are poorly resolved. This study characterized three newly obtained complete mitogenomes of Mekong River catfishes from Vietnam (Pangasius mekongensis, Pangasius krempfi, and Pangasianodon hypophthalmus), as well as the inter- and intrafamilial relationships of the Pangasiidae and catfish families in Siluroidei. The genomic features of their mitogenomes were similar to those of previously reported pangasiids, including all regulatory elements, extended terminal associated sequences (ETAS), and conserved sequence blocks (CSBs) (CSB-1, CSB-2, CSB-3, and CSBs, A to F) in the control region. A comprehensive phylogeny constructed from datasets of multiple 13 PCG sequences from 117 complete mitogenomes of 32 recognized siluriform families established Pangasiidae as monophyletic and a sister group of Austroglanididae. The [Pangasiidae + Austroglanididae] + (Ictaluridae + Cranoglanididae) + Ariidae] clade is a sister to the “Big Africa” major clade of Siluriformes. Furthermore, both phylogenies constructed from the single barcodes (83 partial cox1 and 80 partial cytB, respectively) clearly indicate genus relationships within Pangasiidae. Pangasianodon was monophyletic and a sister to the (Pangasius + Helicophagus + Pseudolais) group. Within the genus Pangasius, P. mekongensis was placed as a sister taxon to P. pangasius. Pangasius sanitwongsei was found to be related to and grouped with Pangasianodon, but in single-gene phylogenies, it was assigned to the Pangasius + Helicophagus + Pseudolais group. The datasets in this study are useful for studying pangasiid systematics, taxonomy and evolution.

Key words: Pangasius, Pangasianodon, Pangasiidae, Mitogenomes, Mitophylogenetic analysis

Citation: Duong TY, Pham LTK, Le XTK, Nguyen NTT, Nor SAM, Le TH. 2022. Mitophylogeny of pangasiid catfishes and its taxonomic implications for Pangasiidae and the suborder Siluroidei. Zool Stud 62:48.

Supplementary materials: Fig. S1Table S1Table S2Table S3Table S4Table S5Table S6