Vol. 60, 2021 (update: 2021.04.12; 07.02) Population Genetics Analysis of a Pomacea Snail (Gastropoda: Ampullariidae) in Thailand and its Low Infection by Angiostrongylus cantonensisAbdulhakam
Dumidae1, Pichamon Janthu1, Chanakan Subkrasae1,
Raxsina Polseela1, Bandid Mangkit2, Aunchalee
Thanwisai1,3,4, and Apichat Vitta1,3,4,* doi:10.6620/ZS.2021.60-31
1Department
of Microbiology and Parasitology, Faculty of Medical Science, Naresuan
University, Phitsanulok 65000, Thailand. E-mail: atp1951@hotmail.com
(Dumidae); pichamonj56@email.nu.ac.th (Janthu); c.chanajj@gmail.com
(Subkrasae); raxsinap@nu.ac.th (Polseela) Received 3 October 2020 / Accepted 1
April 2021 Pomacea
is a freshwater snail in family Ampullariidae that is native to South
and Central America. This snail is among the more important
intermediate hosts for Angiostrongylus
cantonensis and agricultural pests. Herein, we investigated the
prevalence of A. cantonensis
larvae and the genetic diversity of Pomacea
samples collected across Thailand based on mitochondrial cytochrome c oxidase subunit I (COI) gene sequences. The
larval-infection rate was 1.7% in Pomacea
canaliculata specimens collected from the Uttaradit Province of
northern Thailand. We randomly selected specimens of P. canaliculata and P. maculata for genetic analysis.
We analyzed 244 COI
sequences, including 49 sequences from samples collected from Thailand
and a publicly accessible database of snails in their native and
non-native ranges. A maximum-likelihood tree of P. canaliculata and P. maculata revealed two main
clades. The genetic diversity analysis identified seven P. canaliculata haplotypes and six P. maculata haplotypes, and showed
genetic differences between the populations of P. canaliculata and P. maculata. The haplotype networks of
P. canaliculata and P. maculata
populations in Thailand are similar to those of populations in multiple
countries, indicating that this species spread widely to many parts of
the world. Key words: Angiostrongylus, Genetic diversity, Phylogeny, Haplotype, Pomacea. Citation:
Dumidae A, Janthu P, Subkrasae C, Polseela R, Mangkit B, Thanwisai A,
Vitta A. 2021. Population genetics analysis of a Pomacea snail (Gastropoda:
Ampullariidae) in Thailand and its low infection by Angiostrongylus cantonensis. Zool
Stud 60:31. doi:10.6620/ZS.2021.60-31. Supplementary Materials: Fig. S1 | Table S1 | Table S2 | Table S3 | Table S4 | Table S5 | Table S6 | Table S7 |