Vol. 62-30, 2023
Assessing the taxonomic status of the GrayBrocket Mazama simplicicornis argentina Lönnberg, 1919 (Artiodactyla: Cervidae)
Eluzai Dinai Pinto Sandoval
Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE), Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista (UNESP), Jaboticabal-SP, Brazil.
Gabrielle Queiroz Vacari
Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE), Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista (UNESP), Jaboticabal-SP, Brazil.
Juan Pablo Juliá
Facultad de Ciencias Naturales. Universidad Nacional de Tucumán, Argentina.
Susana González
Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Miluse Vozdova
Veterinary Research Institute, Brno, Czech Republic.
Halina Cernohorska
Veterinary Research Institute, Brno, Czech Republic.
Svatava Kubickova
Veterinary Research Institute, Brno, Czech Republic.
Daniela C. Kalthoff
Swedish Museum of Natural History, Frescativägen 40, Stockholm, Sweden.
José Mauricio Barbanti Duarte
Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE), Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista (UNESP), Jaboticabal-SP, Brazil.
Communicated by Jian-Nan Liu
Mazama simplicicornis argentina is the name that was given to describe a gray brocket collected by Lönberg in 1919 in the central Chaco region of Argentina. Subsequent authors, based on morphological similarities, considered this name to be a synonym for the species Subulo gouazoubira Fischer, 1814 from Paraguay. In the absence of genetic analyses to compare the Argentinian and Paraguayan gray brockets,
we aimed to clarify the taxonomy of M. simplicicornis argentina through an integrative assessment using morphological, cytogenetical, and molecular data from its holotype and a current topotype. Qualitative skull features and cranio-morphometric results of M. simplicicornis argentina showed a great similarity with the S. gouazoubira neotype characters. The diploid chromosome number of M. simplicicornis argentina topotype corresponded with the karyotypical pattern of S. gouazoubira with 2n = 70 and FN = 70, showing a great similarity in all classic and molecular cytogenetic results and revealing the homologies between karyotypes. The phylogenetic analysis of mitochondrial genes used in this study (concatenated partial ND5 and Cytb gene) allocated the M. simplicicornis argentina specimens in the monophyletic clade of
S. gouazoubira with a branch value of 100%. These results show that there is no discontinuity between the Argentinian and Paraguayan gray brockets. Therefore, the individuals originally described as M. simplicicornis argentina should be recognized as S. gouazoubira.
Keywords
Animal cytogenetic, Cervidae, Gray brocket, Mitochondrial DNA, Morphology
Supplementary materials
FISH results using BAC clones derived from cattle on
Mazama simplicicornis argentina (MSA) chromosomes.(A) X chromosome labeled with BAC40H2 (red) and BACs 453C5 and 326C13 (green). (B) X chromosome labeled with BAC159O16 (red). (C) Hybridization with the BAC clones derived from the BTA1 (see the RESULTS).
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External body measurements (in mm) of a male
Mazama simplicicornis argentina (Lönnberg, 1919) topotype T450.
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Skull measurement of a male topotype of
Mazama simplicicornis argentina Lönnberg, 1919, and other neotropical cervids.
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BAC clones 1 and X from the CHORI-240 cattle (BTA) library tested in
Mazama simplicicornis argentinaLönnberg, 1919.
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Homologies between
Mazama simplicicornis argentina (MSA),
S. gouazoubira (SGO) chromosomes (Bernegossi et al. 2022), and cattle BTA chromosomes.
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