A total of forty-five isozymes scored from twenty-five enzyme systems in five species of the subfamily Sparinae were detected. Their distribution in twelve tissues was also delineated. The results of the present study show that there are no differences in tissue distribution among these five species, and the patterns of tissue distribution of most isozymes are similar to those of most bony fishes. From the results of the present study, the most appropriate isozymes, tissues, and buffer systems for electrophoretic analysis in future studies of interspecific phylogenetic relationships and intraspecific population genetics of fishes within the subfamily Sparinae will be determined.


