Sérgio L.S. Bueno                                                
 
                                                Department of Zoology, Institute of Biosciences (IB-USP), University of São Paulo, Brazil. Rua do Matão, n. 101, São Paulo 05508-090, Brazil
                                                                        
                                                                        
                     
                                        
                        Jéssica Colavite                                                
 
                                                Department of Zoology, Institute of Biosciences (IB-USP), University of São Paulo, Brazil. Rua do Matão, n. 101, São Paulo 05508-090, Brazil
                                                 Museum of Zoology, University of São Paulo, Av. Nazareth 481, São Paulo, Brazil
                                                                        
                                                                        
                     
                                        
                        Ericson C. Igual                                                
 
                                                Pierre Martin Speleological Group (GPME), Av. Miguel Stefano, 1194, São Paulo (SP), Brazil
                                                                        
                                                                        
                     
                                        
                        Luiz Sérgio F. Martins                                                
 
                                                National Center for Research and Conservation of Continental Aquatic Biodiversity (CEPTA), Chico Mendes Institute for Biodiversity Conservation (ICMBio), Ministry of Environment and Climate Change (MMA), Rodovia SP 201 “Prefeito Euberto Nemésio Pereira de Godoy” km 7.5, Cachoeira de Emas, 13641-001, Pirassununga (SP), Brazil
                                                                        
                                                                        
                     
                                        
                        Jeniffer N. Teles                                                
 
                                                Laboratory of Bioecology and Crustacean Systematics (LBSC), Department of Biology, Faculty of Philosophy, Science and Letters at Ribeirão Preto (FFCLRP), University of São Paulo (USP), Av. Bandeirantes 3900, 14040-901, Ribeirão Preto (SP), Brazil
                                                                        
                                                                        
                     
                                        
                        Marcos Tavares                                                
 
                                                Museum of Zoology, University of São Paulo, Av. Nazareth 481, São Paulo, Brazil
                                                                        
                                                                        
                     
                                        
                        Fernando L. Mantelatto                                                
 
                                                Laboratory of Bioecology and Crustacean Systematics (LBSC), Department of Biology, Faculty of Philosophy, Science and Letters at Ribeirão Preto (FFCLRP), University of São Paulo (USP), Av. Bandeirantes 3900, 14040-901, Ribeirão Preto (SP), Brazil
                                                                        
                                                                        
                     
                    
                                        urn:lsid:zoobank.org:pub:9DDEF627-4BEE-4B0D-86FB-B3C7729173B2
                                                            Communicated by Hsi-Te Shih
                                     
                
                    Two new species of Aegla, A. tamanduatei sp. nov. and A. curucutu sp. nov., are described from low-order streams from Alto Tietê microbasin, headwater section of the Tietê River watershed. Description and validation of the new species are based on morphological and molecular features. Posterior probability and maximum likelihood bootstrap values strongly support the two new species as sister groups. Molecular data and the sharing of notable combination of morphological traits suggest a coherent phylogenetic and distributional relationship among all valid species of Aegla from the Tietê River watershed, save Aegla perobae. A key to all presently known aeglid species from the Tietê River watershed is provided.