Two new species of Aegla, A. tamanduatei sp. nov. and A. curucutu sp. nov., are described from low-order streams from Alto Tietê microbasin, headwater section of the Tietê River watershed. Description and validation of the new species are based on morphological and molecular features. Posterior probability and maximum likelihood bootstrap values strongly support the two new species as sister groups. Molecular data and the sharing of notable combination of morphological traits suggest a coherent phylogenetic and distributional relationship among all valid species of Aegla from the Tietê River watershed, save Aegla perobae. A key to all presently known aeglid species from the Tietê River watershed is provided.
Aeglidae, Crustacea, Integrative taxonomy, Phylogeny, Tietê River watershed
